Pubblicazioni

Autori:

Lampis, Silvia; Zonaro, Emanuele; Bertolini, Cristina; Cecconi, Daniela; Monti, Francesca; Micaroni, Massimo; Turner, Raymond J.; Butler, Clive S.; Vallini, Giovanni

Titolo:

Selenite biotransformation and detoxification by Stenotrophomonas maltophilia SeITE02: Novel clues on the route to bacterial biogenesis of selenium nanoparticles

Anno:

2017

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

Elettronico

Referee:

Sì

Nome rivista:

JOURNAL OF HAZARDOUS MATERIALS

ISSN Rivista:

0304-3894

Intervallo pagine:

3-14

Parole chiave:

Alcohol dehydrogenase; Biogenic selenium nanoparticles; Bioremediation; Selenite reduction; Stenotrophomonas maltophilia SeITE02

Breve descrizione dei contenuti:

A putative biosynthetic mechanism for selenium nanoparticles (SeNPs) and efficient reduction of selenite (SeO32-) in the bacterial strain Stenotrophomonas maltophilia SeITE02 are addressed here on the basis of information gained by a combined approach relying on a set of physiological, chemical/biochemical, microscopy, and proteomic analyses. S. maltophilia SeITE02 is demonstrated to efficiently transform selenite into elemental selenium (Se°) by reducing 100% of 0.5mM of this toxic oxyanion to Se° nanoparticles within 48h growth, in liquid medium. Since the selenite reducing activity was detected in the cytoplasmic protein fraction, while biogenic SeNPs showed mainly extracellular localization, a releasing mechanism of SeNPs from the intracellular environment is hypothesized. SeNPs appeared spherical in shape and with size ranging from 160nm to 250nm, depending on the age of the cultures. Proteomic analysis carried out on the cytoplasmic fraction identified an alcohol dehydrogenase homolog, conceivably correlated with the biogenesis of SeNPs. Finally, by Fourier Transformed Infrared Spectrometry, protein and lipid residues were detected on the surface of biogenic SeNPs. Eventually, this strain might be efficaciously exploited for the remediation of selenite-contaminated environmental matrices due to its high SeO32- reducing efficiency. Biogenic SeNPs may also be considered for technological applications in different fields.

Pagina Web:

http://www.sciencedirect.com/science/article/pii/S0304389416301625

Id prodotto:

92878

Handle IRIS:

11562/947860

ultima modifica:

18 novembre 2022

Citazione bibliografica:

Lampis, Silvia; Zonaro, Emanuele; Bertolini, Cristina; Cecconi, Daniela; Monti, Francesca; Micaroni, Massimo; Turner, Raymond J.; Butler, Clive S.; Vallini, Giovanni, Selenite biotransformation and detoxification by Stenotrophomonas maltophilia SeITE02: Novel clues on the route to bacterial biogenesis of selenium nanoparticles «JOURNAL OF HAZARDOUS MATERIALS» , 2017 , pp. 3-14

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